Cloned (Comment) | Organism |
---|---|
gene ahlD, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha, BL21(DE3) as His-tagged enzyme, and JM109 | Arthrobacter sp. |
gene ahlK, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Klebsiella pneumoniae |
General Stability | Organism |
---|---|
the enzyme is instable during purification | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE | Arthrobacter sp. |
36000 | - |
x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE | Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arthrobacter sp. | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | ? | - |
? | |
additional information | Klebsiella pneumoniae | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | ? | - |
? | |
additional information | Arthrobacter sp. IBN110 | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | ? | - |
? | |
additional information | Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | ? | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | Arthrobacter sp. | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | Klebsiella pneumoniae | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | Arthrobacter sp. IBN110 | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria | N-acyl-(S)-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | Q7X3T2 | gene ahlD | - |
Arthrobacter sp. IBN110 | Q7X3T2 | gene ahlD | - |
Klebsiella pneumoniae | Q7X477 | gene ahlK | - |
Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | Q7X477 | gene ahlK | - |
Purification (Comment) | Organism |
---|---|
partially from strain IBN110 by ammonium sulfate fractionation and two steps of ion exchange chromatography, full purification failed due to enzyme instability | Arthrobacter sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine | hydrolysis of the lactone ring | Arthrobacter sp. | |
an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine | hydrolysis of the lactone ring | Klebsiella pneumoniae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:N-3-oxododecanoyl-L-homoserine lactone/ammonium chloride-grown cell | - |
Arthrobacter sp. | - |
culture condition:N-3-oxododecanoyl-L-homoserine lactone/ammonium chloride-grown cell | - |
Klebsiella pneumoniae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | Arthrobacter sp. | ? | - |
? | |
additional information | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | Klebsiella pneumoniae | ? | - |
? | |
additional information | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | Arthrobacter sp. IBN110 | ? | - |
? | |
additional information | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | ? | - |
? | |
N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-3-oxododecanoyl-L-homoserine | - |
? | |
N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. IBN110 | N-3-oxododecanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Klebsiella pneumoniae | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 | Arthrobacter sp. | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria | Klebsiella pneumoniae | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 | Arthrobacter sp. IBN110 | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria | Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | N-acyl-(S)-homoserine | - |
? | |
N-butanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-butanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-decanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. IBN110 | N-decanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Klebsiella pneumoniae | N-hexanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-hexanoyl-L-homoserine | product analysis of recombinant enzyme expressed in Escherichia coli strain JM109 | ? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. IBN110 | N-hexanoyl-L-homoserine | product analysis of recombinant enzyme expressed in Escherichia coli strain JM109 | ? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | N-hexanoyl-L-homoserine | - |
? | |
N-octanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-octanoyl-L-homoserine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE | Arthrobacter sp. |
More | AhlD contains the conserved HXDH-H-D motif required for full enzyme activity | Arthrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
acyl homoserine degrading enzyme | - |
Arthrobacter sp. |
AHL-degrading enzyme | - |
Arthrobacter sp. |
AHL-degrading enzyme | - |
Klebsiella pneumoniae |
AHLase | - |
Arthrobacter sp. |
AHLase | - |
Klebsiella pneumoniae |
AhlD | - |
Arthrobacter sp. |
AhlK | - |
Klebsiella pneumoniae |
N-acylhomoserine lactonase | - |
Arthrobacter sp. |
N-acylhomoserine lactonase | - |
Klebsiella pneumoniae |